rna sequencing libraries Search Results


rna library sequencing  (Illumina Inc)
99
Illumina Inc rna library sequencing
Rna Library Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna sequencing libraries  (Novogene)
90
Novogene rna sequencing libraries
Rna Sequencing Libraries, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequencing libraries for starr-seq plasmid, transfected dna and transcribed rna reporters  (Illumina Inc)
90
Illumina Inc sequencing libraries for starr-seq plasmid, transfected dna and transcribed rna reporters
Sequencing Libraries For Starr Seq Plasmid, Transfected Dna And Transcribed Rna Reporters, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq library preparation and sequencing  (Beijing Genomics Institute Shenzhen)
90
Beijing Genomics Institute Shenzhen rna-seq library preparation and sequencing
Rna Seq Library Preparation And Sequencing, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rip-seq (illumina/solexa rna sequencing) cdna library construction  (Illumina Inc)
90
Illumina Inc rip-seq (illumina/solexa rna sequencing) cdna library construction
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Rip Seq (Illumina/Solexa Rna Sequencing) Cdna Library Construction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poly (a) + rna  (Illumina Inc)
90
Illumina Inc poly (a) + rna
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Poly (A) + Rna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq library preparation and sequencing of ovarian samples (n = 4 rats per group)  (Novogene)
90
Novogene rna-seq library preparation and sequencing of ovarian samples (n = 4 rats per group)
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Rna Seq Library Preparation And Sequencing Of Ovarian Samples (N = 4 Rats Per Group), supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna extractions, cdna synthesis library preparation and sequencing  (LGC Genomics GmbH)
90
LGC Genomics GmbH rna extractions, cdna synthesis library preparation and sequencing
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Rna Extractions, Cdna Synthesis Library Preparation And Sequencing, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna libraries transcriptome sequencing clustering  (Novogene)
90
Novogene rna libraries transcriptome sequencing clustering
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Rna Libraries Transcriptome Sequencing Clustering, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna extraction and library preparation  (Novogene)
90
Novogene rna extraction and library preparation
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Rna Extraction And Library Preparation, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna library construction and rna sequencing illumina 3000  (Illumina Inc)
90
Illumina Inc cdna library construction and rna sequencing illumina 3000
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Cdna Library Construction And Rna Sequencing Illumina 3000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strand-specific rna-seq library construction and sequencing  (Novogene)
90
Novogene strand-specific rna-seq library construction and sequencing
LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 <t>RIP-Seq</t> targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).
Strand Specific Rna Seq Library Construction And Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 RIP-Seq targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).

Journal: Molecular and Cellular Biology

Article Title: LIN28A Modulates Splicing and Gene Expression Programs in Breast Cancer Cells

doi: 10.1128/MCB.00426-15

Figure Lengend Snippet: LIN28 binds novel mRNAs in human breast cancer cells. (A) Genomic distribution of the 843 LIN28-bound genes. LIN28 is enriched predominantly in the coding exons (CDS) and 3′ UTRs of the target mRNAs. (B) GGAGA prevalence in LIN28-bound and unbound genes. The box plot shows the distribution of normalized GGAGA counts for LIN28-bound and unbound genes. The LIN28-bound genes are those that overlap the 843 RIP-Seq targets, and the unbound genes are those that do not overlap the RIP-Seq targets. Data are reported as normalized GGAGA counts per kilobase of exon; the P value was determined by a one-sided Wilcoxon rank sum test. (C) Read coverage of a subset of LIN28-bound targets. We normalized the coverage to reads per million mapped reads. These genes include only those identified in MCF-7M cells, including the S100 calcium-binding protein A9 (S100A9), lysophospholipase-like 1 (LYPLAL1), ubiquinol-cytochrome c reductase hinge protein (UQCRH), and O-6-methylguanine-DNA methyltransferase (MGMT) genes, and those identified in both MCF-7M and ES cells (5, 24), including the charged multivesicular body protein 2A (CHMP2A) and RNA-binding motif protein 3 (RBM3) genes. The fold enrichment for LIN28 IP compared to the control IP (CTL-IP) for each target is indicated. (D) LIN28 binding of a subset of targets. RNA protein complexes were isolated from cells by using an antibody against LIN28 or normal rabbit serum IgG (CTL), followed by RNA extraction and quantitative real-time PCR analysis. A subset of mRNAs identified by RIP-Seq was analyzed. Data for LIN28 are represented as fold enrichments of mRNAs present in the LIN28 IP relative to the control IP. The experiment was done in 2 biological replicates. Data from a representative experiment are shown. (E) mRNA expression of a subset of LIN28-bound targets. Total RNA was isolated, and the expression of the subset of genes described above for panel C was analyzed by qRT-PCR. Total relative mRNA levels were normalized against the RPL13A level. Values are means ± standard deviations (n = 3).

Article Snippet: RIP-Seq (Illumina/Solexa RNA sequencing) cDNA library construction.

Techniques: Binding Assay, RNA Binding Assay, Isolation, RNA Extraction, Real-time Polymerase Chain Reaction, Expressing, Quantitative RT-PCR